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Wednesday, April 26 • 12:30pm - 2:00pm
Using hormone treatments to regulate gene expression of MUPs in HEPA1-6 cells

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The genome of the house mouse, Mus musculus, contains 21 major urinary proteins (MUPs). A given mouse will express and emit a certain subset of MUPs, which can then be used to identify individual mice. The mechanism by which particular MUPs are chosen for expression is not clearly understood. This study looks at the regulation in gene expression of the MUPs. Hormone treatments on a cultured mouse liver cell line were used to induce gene expression. HEPA1-6 cells were treated with testosterone, dihydrotestosterone, growth hormone and thyroxine individually or in combination. Alternatively, inhibition of methylation enzymes was used as a mechanism to “turn on” gene expression of the MUPs. Polymerase chain reaction (PCR) and gel electrophoresis were used to study MUP expression at the RNA level. The MUPs present a model system for studying gene regulation, and contributes to the greater understanding of one of molecular biology’s long standing questions.


Wednesday April 26, 2017 12:30pm - 2:00pm PDT
Concourse - Wilma Sherrill Center