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Wednesday, April 26 • 12:30pm - 2:00pm
Deorphanization of Vomeronasal Type-2 Receptors

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The vomeronasal organ (VNO) is an olfactory sense organ located in the nose of mice that detects pheromone signals through ligand binding to G-protein coupled receptors. There are three families of VNO receptors, V1R, V2R, and FPR. V2Rs in mice primarily serve to bind large molecules like the major urinary proteins (MUPs), proteins secreted in urine that trigger contextual behaviors in the recipient. Through combinatorial coding, multiple combinations of MUPs can activate multiple V2Rs in different ways, leading to complex signals based on a small library of ligands. This research sets out to deorphanize V2Rs and pair them with their cognate ligands to create a library of receptor-ligand pairings. Receptor primers are amplified through PCR, then cloned into E. coli expression vectors. Expression vectors are then placed into cell lines, and GPCR activity is analyzed using patch clamp. Ligands are introduced to patched cells, and membrane depolarization indicates a successful match of receptor and ligand. These experiments are an important first step to being able to better understand and map the exact neural pathways activated by an environmental chemical stimulus, and how it produces a response in the host.

Wednesday April 26, 2017 12:30pm - 2:00pm PDT
Concourse - Wilma Sherrill Center

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