American ginseng (Panax quinquefolius L.) is a threatened perennial understory plant endemic to eastern deciduous forests. The plant is harvested and sold on the Asian markets for its secondary metabolites, ginsenosides, which give it its medicinal qualities. Information on phytochemical profiles of populations would give more insight on creating cultivars labeled for specific medicinal properties, ideally reducing the demand for wild harvested ginseng. Genetic diversity of ginseng is thought to be more widespread in the Appalachian region, due to the glacial refugia created during the Pleistocene epoch. Ginsenoside profile diversity may also be more widespread in the Appalachian region and may be linked to genetic diversity. We analyzed the ginsenoside profiles in 178 roots from 17 NC populations and 2 Virginia populations. Six ginsenosides (Rb1, Rb2, Rg1, Re, Rd, and Rc) were characterized and quantified using methanol-reflux extraction and high performance liquid chromatography (HPLC). Preliminary analysis has confirmed variation in chemotypes with most plants exhibiting RG (Re/Rg1 < 1) chemotypes and only a few populations showing RE (Re/Rg1 > 2) or Intermediate (1 < Re/Eg1 < 2) chemotypes, which could be a result of outplanting commercial seeds.