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Wednesday, April 26 • 10:35am - 10:55am
Using Microsatellite Markers To Determine Levels Of Hybridization Between Pitcher Plants Sarracenia Purpurea Var. Montana And Sarracenia Jonesii

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Pitcher plants (Sarracenia) are a genus of carnivorous plants that live in bogs and wetlands and obtain most of their nitrogen from insect digestion. Pitcher plants freely hybridize in the wild. Twenty-one natural F1 hybrids between various Sarracenia species have been documented, but little is known about later-generation hybrids. Many species of pitcher plants, including Sarracenia jonesii (mountain sweet pitcher plant), are endangered, and there is little published genetic data on pitcher plants. Qiagen kits were used to extract DNA from leaf tissues of 13 different pitcher plant individuals from western North Carolina, which appeared to be hybrids between Sarracenia purpurea var. montana (mountain purple pitcher plant) and S. jonesii. Microsatellite markers were used to determine levels of hybridization, with five microsatellite primer sets used to amplify polymorphic regions of DNA previously found to be specific to either S. purpurea var. montana or S. jonesii. PCR products were run through 2% agarose gels in order to analyze and quantify band sizes. Automated fragment analysis was also used to determine more accurate band lengths. Current results identified multiple individuals as Sarracenia purpurea var. montana and Sarracenia jonesii hybrids, and have shown possible hybridization between S. flava and S. leucophylla. Results from this project will help the United States Fish and Wildlife Service more accurately identify hybrid species and also help with the conservation and protection of pitcher plants.

Wednesday April 26, 2017 10:35am - 10:55am PDT
038 Karpen Hall

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