Anolis lizards in the West Indies are one of the best-studied examples of an adaptive radiation, the evolution of various forms from a single ancestral lineage. Speciation and diversification in the region have led to the present recognition of hundreds of anole species. Green and brown anoles have evolved independently on many of these islands, including Cuba. The Cuban anoles have since spread to other regions, including the Bahamas and the Cayman Islands. Some of these diaspora have diversified such that they are recognized as different species. Within this group, two species occur on Cay Sal Bank Anolis sagrei and Anolis fairchildi. Cay Sal Bank encompasses a small ring of islands located between Florida, USA and Cuba. It is unknown whether these species came from Cuba or the Bahamas and for Anolis farichildi, whether or not it is a valid species. The morphological features, such as Anolis fairchildi large size, suggest that they evolved from a historical colonization from Cuba, yet this is not definitively known, and no evidence suggests when this colonization might have occurred. An alternative is that these species colonized the area from the Bahamas fairly recently. On Grand Cayman, an unknown green anole species has been introduced and using genetic analyses it will be determined where they originated. To test these hypotheses, I have generated genetic data for the mitochondrial locus ND2 from A. fairchildi, A. sagrei, three unknown samples from Grand Cayman, and the related green and brown anole species. I then aligned the resulting sequences with data from all other West Indian anoles and constructed a series of phylogenetic trees to examine relationships among these anoles. Finally, I will estimated the divergence times of these species using calibrated molecular clock analyses.