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Wednesday, April 26 • 2:40pm - 3:00pm
Microbial Isolation, Characterization And Antibiotic Extraction Of Bacterial Strains From The Southwestern United States

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Multidrug resistant bacteria pose a huge threat to human health due to overprescription and misuse of antibiotics, an industry of agriculture reliance, and the decline of novel antibiotic discovery. According to the CDC at least two million people in the United States each year are infected with multidrug resistant bacteria and more than 23,000 succumb to those infections. Natural product (NP) isolation is a robust source of potential antibiotics even though recent random selection of organisms which undergo novel NP biosynthesis is rare. Understanding how ecological and evolutionary pressures drive NP synthesis may lead to new discoveries in novel NP production and isolation. This research involves the isolation and purification of 101 bacterial soil samples from the Southwestern United States and subsequent screening of those bacteria for antibiosis induction through coculture and quadculture agar overlay and lawn inhibition assays. Bacteria found to be strong antibiotic producers were then characterized by 16S ribosomal polymerase chain reactions and subjected to scale up and extraction to isolate the produced antibiotic. Currently antibiotics produced by three bacteria, a Bacillus strain (SS729) and two Streptomyces strains (SS735 and SS746) are being isolated and characterized. SS735 was found to have antibiotic activity against Gram-positive Staphylococcus aureus while the other two strains, SS729 and SS746 were found to have antibiotic activity against both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli in agar overlay inhibition assays.

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Wednesday April 26, 2017 2:40pm - 3:00pm
014 Zeis Hall

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