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Wednesday, April 26 • 1:40pm - 2:00pm
Identification Of Antibacterial Secondary Metabolites Produced By Bacteria Of The Chromobacterium Genus

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Multidrug resistant bacterial infections, which arise due to misuse and overuse of antibiotics, are responsible for many nosocomial infections and are a threat to human health. Derivatization of known antibiotic compounds via total or semisynthesis can be time consuming and ineffective at targeting specific bacteria. This investigation focuses on bacteria found in the phytotelmata of Sarracenia pitcher plants and the natural antibiotic compounds they secrete under varying conditions. The aim of the project is to find single-producer and co-culture producing bacteria that secrete secondary metabolites effective a broad spectrum of Gram-positive and -negative pathogens. A Pseudomonas (CMCP E3) and Chromobacterium (CP2 SSIV) isolated in this study have been found to be effective against Gram-positive bacteria Staphylococcus aureus and the fungi Fusarium solani. The bacteria strains were cultured in minimal media containing either succinate or citrate that showed the densest growth after 72 hours. Final characterization of the CMCP E3 antibacterial compound was inconclusive. A 6 L succinate culture of the CP2 SSIV bacterium yielded on average 16 mg of crude product and 0.5 mg of antibacterial compound. Optimization of antibacterial isolation and characterization of CP2 SSIV secondary metabolite using column chromatography, NMR, and mass spectrometry is ongoing.


Wednesday April 26, 2017 1:40pm - 2:00pm PDT
014 Zeis Hall