2017ASSURC.sched.com has ended
Back To Schedule
Wednesday, April 26 • 11:50am - 12:10pm
Antibiotic Activity Of Natural Products Produced By Bacteria Isolated In Western North Carolina

Sign up or log in to save this to your schedule, view media, leave feedback and see who's attending!

In order to treat the growing number of antibiotic resistant pathogens, the isolation and production of new antibiotics is necessary. The need to discover novel antibiotics is increased by the lack of interest of large pharmaceutical companies. The two strains being studied were isolated from the phytotelma of Serracenia pitcher plants found in Western North Carolina. Pseudomonas CM/CP G1 was grown in a 25 mMol sodium succinate minimal media, and Streptomyces SS568 was grown in both glucose and acetate minimal medias (12.5mMol). Antibiotic activity of crude extractions were tested against Gram positive Staphylococcus aureus (grown on 10% Triptic Soy Agarose plates). The presence of antibiotic compounds has been confirmed for both strains in crude extracts, and compounds isolated from SS568 are currently under test for antibiotic activity against S. aureus. Characterization of active compounds will be completed upon results of activity. Techniques of liquid-liquid extraction and column chromatography are used to purify the active metabolites, which are then characterized by 1D and 2D NMR, IR, and LC-MSMS.


Wednesday April 26, 2017 11:50am - 12:10pm PDT
014 Zeis Hall

Attendees (1)